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human embryonic kidney cell line hek293t  (ATCC)


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    ATCC human embryonic kidney cell line hek293t
    SUR1 protein levels were elevated in the bronchoalveolar lavage fluid (BALF), but not the serum, of sepsis-related ALI patients and CLP-induced ALI mouse models (A) Dot blot analysis of BALF and serum collected from sepsis-related ALI patients or controls. NC (negative control), PBS; PC (positive control), whole-cell protein lysates of <t>HEK293T</t> cells. Control, patients who underwent abdominal surgery without ALI or bacterial infection; ALI, sepsis-associated ALI. Red circle, site of PBS drops. (B) Cytokine levels in BALF and serum of patients determined by a cytokine screening assay. n = 3 for control; n = 9 for ALI. (C) Dot blot analysis of BALF and serum from CLP-induced ALI mice ( n = 6). (D–F) Western blot and RT-qPCR analyses of lung tissue samples from CLP-induced ALI mice. Data are presented as mean ± SD. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, no significance. Data in (A, B, C, and F) were analyzed using Welch’s t test, and data in (E) were analyzed using an unpaired two-tailed Student’s t test. Representative images are shown.
    Human Embryonic Kidney Cell Line Hek293t, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 12193 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+embryonic+kidney+293t+cells/293T%2F17/pmc13673107-38-0-7
    Average 99 stars, based on 12193 article reviews
    human embryonic kidney cell line hek293t - by Bioz Stars, 2026-10
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    Images

    1) Product Images from "Sulfonylurea receptor 1 deficiency alleviates acute lung injury by activating PARP1 in epithelial cells"

    Article Title: Sulfonylurea receptor 1 deficiency alleviates acute lung injury by activating PARP1 in epithelial cells

    Journal: iScience

    doi: 10.1016/j.isci.2026.117627

    SUR1 protein levels were elevated in the bronchoalveolar lavage fluid (BALF), but not the serum, of sepsis-related ALI patients and CLP-induced ALI mouse models (A) Dot blot analysis of BALF and serum collected from sepsis-related ALI patients or controls. NC (negative control), PBS; PC (positive control), whole-cell protein lysates of HEK293T cells. Control, patients who underwent abdominal surgery without ALI or bacterial infection; ALI, sepsis-associated ALI. Red circle, site of PBS drops. (B) Cytokine levels in BALF and serum of patients determined by a cytokine screening assay. n = 3 for control; n = 9 for ALI. (C) Dot blot analysis of BALF and serum from CLP-induced ALI mice ( n = 6). (D–F) Western blot and RT-qPCR analyses of lung tissue samples from CLP-induced ALI mice. Data are presented as mean ± SD. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, no significance. Data in (A, B, C, and F) were analyzed using Welch’s t test, and data in (E) were analyzed using an unpaired two-tailed Student’s t test. Representative images are shown.
    Figure Legend Snippet: SUR1 protein levels were elevated in the bronchoalveolar lavage fluid (BALF), but not the serum, of sepsis-related ALI patients and CLP-induced ALI mouse models (A) Dot blot analysis of BALF and serum collected from sepsis-related ALI patients or controls. NC (negative control), PBS; PC (positive control), whole-cell protein lysates of HEK293T cells. Control, patients who underwent abdominal surgery without ALI or bacterial infection; ALI, sepsis-associated ALI. Red circle, site of PBS drops. (B) Cytokine levels in BALF and serum of patients determined by a cytokine screening assay. n = 3 for control; n = 9 for ALI. (C) Dot blot analysis of BALF and serum from CLP-induced ALI mice ( n = 6). (D–F) Western blot and RT-qPCR analyses of lung tissue samples from CLP-induced ALI mice. Data are presented as mean ± SD. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, no significance. Data in (A, B, C, and F) were analyzed using Welch’s t test, and data in (E) were analyzed using an unpaired two-tailed Student’s t test. Representative images are shown.

    Techniques Used: Dot Blot, Analysis, Negative Control, Positive Control, Control, Infection, Screening, Western Blot, Quantitative RT-PCR, Tissue, Two Tailed Test

    SUR1 interacted with PARP1 to suppress DNA damage repair and induce apoptosis of bronchial epithelial cells (A) Co-immunoprecipitation and western blot analyses of HEK293T cells transfected with FLAG-SUR1 plasmids (left) or human bronchial epithlial (HBE) cells (right). (B) Immunofluorescence staining of HBE cells. Scale bars, 25 μm. (C) Homologous recombination reporter assay in U2OS-DRGFP cells transfected with SUR1 siRNA, followed by flow cytometry analysis. (D) Western blot analysis of HBE cells transfected with SUR1 siRNAs with or without 20 ng/mL TNF-α or 50 μg/mL LPS treatment for 1 h. (E) ROS levels in HBE cells with SUR1 knockdown, treated with or without TNF-α 20 ng/mL for 3 h. (F) Apoptosis assay by flow cytometry in HBE cells transfected with SUR1 siRNAs and treated with TNF-α. Data are presented as mean ± SD ( n = 3). ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001. Co-localization in (B) was assessed using Pearson’s correlation coefficient. Data in (C–F) were analyzed using two-way ANOVA followed by Tukey’s multiple-comparisons test. Representative images are shown.
    Figure Legend Snippet: SUR1 interacted with PARP1 to suppress DNA damage repair and induce apoptosis of bronchial epithelial cells (A) Co-immunoprecipitation and western blot analyses of HEK293T cells transfected with FLAG-SUR1 plasmids (left) or human bronchial epithlial (HBE) cells (right). (B) Immunofluorescence staining of HBE cells. Scale bars, 25 μm. (C) Homologous recombination reporter assay in U2OS-DRGFP cells transfected with SUR1 siRNA, followed by flow cytometry analysis. (D) Western blot analysis of HBE cells transfected with SUR1 siRNAs with or without 20 ng/mL TNF-α or 50 μg/mL LPS treatment for 1 h. (E) ROS levels in HBE cells with SUR1 knockdown, treated with or without TNF-α 20 ng/mL for 3 h. (F) Apoptosis assay by flow cytometry in HBE cells transfected with SUR1 siRNAs and treated with TNF-α. Data are presented as mean ± SD ( n = 3). ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001. Co-localization in (B) was assessed using Pearson’s correlation coefficient. Data in (C–F) were analyzed using two-way ANOVA followed by Tukey’s multiple-comparisons test. Representative images are shown.

    Techniques Used: Co-Immunoprecipitation Assay, Western Blot, Transfection, Immunofluorescence, Staining, Homologous Recombination, Reporter Assay, Flow Cytometry, Analysis, Knockdown, Apoptosis Assay


    Figure Legend Snippet:

    Techniques Used: Cell-Signaling, Recombinant, Transfection, Caspase-3 Activity Assay, Protein Extraction, Knock-Out, Sequencing, Control, Plasmid Preparation, Software, Analysis

    Related Articles

    Cell Culture:

    Article Title: Targeting SHMT2 reverses lactate-driven CAF-mediated T-cell exclusion and sensitizes HNSCC to anti-PD-1 immunotherapy
    Article Snippet: .. Cell culture and culture conditions The human HNSCC cell lines (Fadu and HN8) and the human embryonic kidney 293T cells were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) and the Cell Bank of the Chinese Academy of Cell Death and Disease Sciences (Shanghai, China), respectively. .. The Fadu cell line was maintained in MEM medium (Gibco, catalog #11095080), while the HN8 cell line was cultured in DMEM medium (Gibco, catalog #11965092).

    Sterility:

    Article Title: Language Modeling Materializes a World Model of Protein Biology
    Article Snippet: Background absorbance from wells with coating antigen omitted was subtracted from the raw measurements, and the adjusted measurements plotted with GraphPad Prism. .. To promote cell adhesion, 8-well chamber slides (Cellvis) were coated with 150 μ L/well of Poly-D-Lysine for 1–4 hrs at 37 ◦ C. Following aspiration of the coating solution, wells were washed twice with 200 μ L of sterile phosphate buffered saline (PBS, pH 7.4) and dried in a biosafety cabinet for 10 min. Human embryonic kidney 293T cells (ATCC) were then seeded at a density of 40,000 cells/well in Dulbecco’s modified Eagle’s medium (DMEM, Gibco) supplemented with 10% v/v FBS and allowed to recover overnight in a humidified incubator at 37 ◦ C with 5% CO 2 . ..

    Saline:

    Article Title: Language Modeling Materializes a World Model of Protein Biology
    Article Snippet: Background absorbance from wells with coating antigen omitted was subtracted from the raw measurements, and the adjusted measurements plotted with GraphPad Prism. .. To promote cell adhesion, 8-well chamber slides (Cellvis) were coated with 150 μ L/well of Poly-D-Lysine for 1–4 hrs at 37 ◦ C. Following aspiration of the coating solution, wells were washed twice with 200 μ L of sterile phosphate buffered saline (PBS, pH 7.4) and dried in a biosafety cabinet for 10 min. Human embryonic kidney 293T cells (ATCC) were then seeded at a density of 40,000 cells/well in Dulbecco’s modified Eagle’s medium (DMEM, Gibco) supplemented with 10% v/v FBS and allowed to recover overnight in a humidified incubator at 37 ◦ C with 5% CO 2 . ..

    Hood:

    Article Title: Language Modeling Materializes a World Model of Protein Biology
    Article Snippet: Background absorbance from wells with coating antigen omitted was subtracted from the raw measurements, and the adjusted measurements plotted with GraphPad Prism. .. To promote cell adhesion, 8-well chamber slides (Cellvis) were coated with 150 μ L/well of Poly-D-Lysine for 1–4 hrs at 37 ◦ C. Following aspiration of the coating solution, wells were washed twice with 200 μ L of sterile phosphate buffered saline (PBS, pH 7.4) and dried in a biosafety cabinet for 10 min. Human embryonic kidney 293T cells (ATCC) were then seeded at a density of 40,000 cells/well in Dulbecco’s modified Eagle’s medium (DMEM, Gibco) supplemented with 10% v/v FBS and allowed to recover overnight in a humidified incubator at 37 ◦ C with 5% CO 2 . ..

    Modification:

    Article Title: Language Modeling Materializes a World Model of Protein Biology
    Article Snippet: Background absorbance from wells with coating antigen omitted was subtracted from the raw measurements, and the adjusted measurements plotted with GraphPad Prism. .. To promote cell adhesion, 8-well chamber slides (Cellvis) were coated with 150 μ L/well of Poly-D-Lysine for 1–4 hrs at 37 ◦ C. Following aspiration of the coating solution, wells were washed twice with 200 μ L of sterile phosphate buffered saline (PBS, pH 7.4) and dried in a biosafety cabinet for 10 min. Human embryonic kidney 293T cells (ATCC) were then seeded at a density of 40,000 cells/well in Dulbecco’s modified Eagle’s medium (DMEM, Gibco) supplemented with 10% v/v FBS and allowed to recover overnight in a humidified incubator at 37 ◦ C with 5% CO 2 . ..

    Article Title: Computational design of alpha(v) beta (6) integrin binding proteins
    Article Snippet: .. In Vitro Binding Assays Using Fluorescently Labelled Binders: Epidermoid cancer cells (A431) and human embryonic kidney 293T cells (HEK 293T) were purchased from American Type Culture Collection (ATCC) and grown in Dulbecco's Modified Eagle Medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) in a humidified atmosphere with 5% CO2 at 37° C. Binding assays were performed on A431 carcinoma cells. .. A431 cells were dissociated from culture flasks with enzyme-free cell dissociation buffer (Gibco).

    Transfection:

    Article Title: GATA1 repression-mediated ADRB2/cAMP/PKA signaling activation underlies the antidepressant effects of targeted geniposide delivery.
    Article Snippet: .. Human embryonic kidney 293T cells (ATCC, USA) were transfected with either control plasmids or GATA1 overexpression plasmids (GenePharma, China), forming oe-NC and oe-GATA1 groups. .. AR TI CL E IN P RE SS After 48 h, cells were fixed with 0.75% formaldehyde (Sigma-Aldrich, USA) for 10 min, and the reaction was quenched with 125 mM glycine.

    Control:

    Article Title: GATA1 repression-mediated ADRB2/cAMP/PKA signaling activation underlies the antidepressant effects of targeted geniposide delivery.
    Article Snippet: .. Human embryonic kidney 293T cells (ATCC, USA) were transfected with either control plasmids or GATA1 overexpression plasmids (GenePharma, China), forming oe-NC and oe-GATA1 groups. .. AR TI CL E IN P RE SS After 48 h, cells were fixed with 0.75% formaldehyde (Sigma-Aldrich, USA) for 10 min, and the reaction was quenched with 125 mM glycine.

    Over Expression:

    Article Title: GATA1 repression-mediated ADRB2/cAMP/PKA signaling activation underlies the antidepressant effects of targeted geniposide delivery.
    Article Snippet: .. Human embryonic kidney 293T cells (ATCC, USA) were transfected with either control plasmids or GATA1 overexpression plasmids (GenePharma, China), forming oe-NC and oe-GATA1 groups. .. AR TI CL E IN P RE SS After 48 h, cells were fixed with 0.75% formaldehyde (Sigma-Aldrich, USA) for 10 min, and the reaction was quenched with 125 mM glycine.

    In Vitro:

    Article Title: Computational design of alpha(v) beta (6) integrin binding proteins
    Article Snippet: .. In Vitro Binding Assays Using Fluorescently Labelled Binders: Epidermoid cancer cells (A431) and human embryonic kidney 293T cells (HEK 293T) were purchased from American Type Culture Collection (ATCC) and grown in Dulbecco's Modified Eagle Medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) in a humidified atmosphere with 5% CO2 at 37° C. Binding assays were performed on A431 carcinoma cells. .. A431 cells were dissociated from culture flasks with enzyme-free cell dissociation buffer (Gibco).

    Binding Assay:

    Article Title: Computational design of alpha(v) beta (6) integrin binding proteins
    Article Snippet: .. In Vitro Binding Assays Using Fluorescently Labelled Binders: Epidermoid cancer cells (A431) and human embryonic kidney 293T cells (HEK 293T) were purchased from American Type Culture Collection (ATCC) and grown in Dulbecco's Modified Eagle Medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) in a humidified atmosphere with 5% CO2 at 37° C. Binding assays were performed on A431 carcinoma cells. .. A431 cells were dissociated from culture flasks with enzyme-free cell dissociation buffer (Gibco).

    other:

    Article Title: Repurposing the Neurokinin-1 Receptor Antagonist Aprepitant to Eradicate Relapsed/Refractory Acute Lymphoblastic Leukemia via Mitochondria-Driven Necroptosis.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.



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    SUR1 protein levels were elevated in the bronchoalveolar lavage fluid (BALF), but not the serum, of sepsis-related ALI patients and CLP-induced ALI mouse models (A) Dot blot analysis of BALF and serum collected from sepsis-related ALI patients or controls. NC (negative control), PBS; PC (positive control), whole-cell protein lysates of <t>HEK293T</t> cells. Control, patients who underwent abdominal surgery without ALI or bacterial infection; ALI, sepsis-associated ALI. Red circle, site of PBS drops. (B) Cytokine levels in BALF and serum of patients determined by a cytokine screening assay. n = 3 for control; n = 9 for ALI. (C) Dot blot analysis of BALF and serum from CLP-induced ALI mice ( n = 6). (D–F) Western blot and RT-qPCR analyses of lung tissue samples from CLP-induced ALI mice. Data are presented as mean ± SD. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, no significance. Data in (A, B, C, and F) were analyzed using Welch’s t test, and data in (E) were analyzed using an unpaired two-tailed Student’s t test. Representative images are shown.
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    SUR1 protein levels were elevated in the bronchoalveolar lavage fluid (BALF), but not the serum, of sepsis-related ALI patients and CLP-induced ALI mouse models (A) Dot blot analysis of BALF and serum collected from sepsis-related ALI patients or controls. NC (negative control), PBS; PC (positive control), whole-cell protein lysates of <t>HEK293T</t> cells. Control, patients who underwent abdominal surgery without ALI or bacterial infection; ALI, sepsis-associated ALI. Red circle, site of PBS drops. (B) Cytokine levels in BALF and serum of patients determined by a cytokine screening assay. n = 3 for control; n = 9 for ALI. (C) Dot blot analysis of BALF and serum from CLP-induced ALI mice ( n = 6). (D–F) Western blot and RT-qPCR analyses of lung tissue samples from CLP-induced ALI mice. Data are presented as mean ± SD. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, no significance. Data in (A, B, C, and F) were analyzed using Welch’s t test, and data in (E) were analyzed using an unpaired two-tailed Student’s t test. Representative images are shown.
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    Image Search Results


    SUR1 protein levels were elevated in the bronchoalveolar lavage fluid (BALF), but not the serum, of sepsis-related ALI patients and CLP-induced ALI mouse models (A) Dot blot analysis of BALF and serum collected from sepsis-related ALI patients or controls. NC (negative control), PBS; PC (positive control), whole-cell protein lysates of HEK293T cells. Control, patients who underwent abdominal surgery without ALI or bacterial infection; ALI, sepsis-associated ALI. Red circle, site of PBS drops. (B) Cytokine levels in BALF and serum of patients determined by a cytokine screening assay. n = 3 for control; n = 9 for ALI. (C) Dot blot analysis of BALF and serum from CLP-induced ALI mice ( n = 6). (D–F) Western blot and RT-qPCR analyses of lung tissue samples from CLP-induced ALI mice. Data are presented as mean ± SD. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, no significance. Data in (A, B, C, and F) were analyzed using Welch’s t test, and data in (E) were analyzed using an unpaired two-tailed Student’s t test. Representative images are shown.

    Journal: iScience

    Article Title: Sulfonylurea receptor 1 deficiency alleviates acute lung injury by activating PARP1 in epithelial cells

    doi: 10.1016/j.isci.2026.117627

    Figure Lengend Snippet: SUR1 protein levels were elevated in the bronchoalveolar lavage fluid (BALF), but not the serum, of sepsis-related ALI patients and CLP-induced ALI mouse models (A) Dot blot analysis of BALF and serum collected from sepsis-related ALI patients or controls. NC (negative control), PBS; PC (positive control), whole-cell protein lysates of HEK293T cells. Control, patients who underwent abdominal surgery without ALI or bacterial infection; ALI, sepsis-associated ALI. Red circle, site of PBS drops. (B) Cytokine levels in BALF and serum of patients determined by a cytokine screening assay. n = 3 for control; n = 9 for ALI. (C) Dot blot analysis of BALF and serum from CLP-induced ALI mice ( n = 6). (D–F) Western blot and RT-qPCR analyses of lung tissue samples from CLP-induced ALI mice. Data are presented as mean ± SD. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, no significance. Data in (A, B, C, and F) were analyzed using Welch’s t test, and data in (E) were analyzed using an unpaired two-tailed Student’s t test. Representative images are shown.

    Article Snippet: Human embryonic kidney cell line HEK293T , American Type Culture Collection , N/A.

    Techniques: Dot Blot, Analysis, Negative Control, Positive Control, Control, Infection, Screening, Western Blot, Quantitative RT-PCR, Tissue, Two Tailed Test

    SUR1 interacted with PARP1 to suppress DNA damage repair and induce apoptosis of bronchial epithelial cells (A) Co-immunoprecipitation and western blot analyses of HEK293T cells transfected with FLAG-SUR1 plasmids (left) or human bronchial epithlial (HBE) cells (right). (B) Immunofluorescence staining of HBE cells. Scale bars, 25 μm. (C) Homologous recombination reporter assay in U2OS-DRGFP cells transfected with SUR1 siRNA, followed by flow cytometry analysis. (D) Western blot analysis of HBE cells transfected with SUR1 siRNAs with or without 20 ng/mL TNF-α or 50 μg/mL LPS treatment for 1 h. (E) ROS levels in HBE cells with SUR1 knockdown, treated with or without TNF-α 20 ng/mL for 3 h. (F) Apoptosis assay by flow cytometry in HBE cells transfected with SUR1 siRNAs and treated with TNF-α. Data are presented as mean ± SD ( n = 3). ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001. Co-localization in (B) was assessed using Pearson’s correlation coefficient. Data in (C–F) were analyzed using two-way ANOVA followed by Tukey’s multiple-comparisons test. Representative images are shown.

    Journal: iScience

    Article Title: Sulfonylurea receptor 1 deficiency alleviates acute lung injury by activating PARP1 in epithelial cells

    doi: 10.1016/j.isci.2026.117627

    Figure Lengend Snippet: SUR1 interacted with PARP1 to suppress DNA damage repair and induce apoptosis of bronchial epithelial cells (A) Co-immunoprecipitation and western blot analyses of HEK293T cells transfected with FLAG-SUR1 plasmids (left) or human bronchial epithlial (HBE) cells (right). (B) Immunofluorescence staining of HBE cells. Scale bars, 25 μm. (C) Homologous recombination reporter assay in U2OS-DRGFP cells transfected with SUR1 siRNA, followed by flow cytometry analysis. (D) Western blot analysis of HBE cells transfected with SUR1 siRNAs with or without 20 ng/mL TNF-α or 50 μg/mL LPS treatment for 1 h. (E) ROS levels in HBE cells with SUR1 knockdown, treated with or without TNF-α 20 ng/mL for 3 h. (F) Apoptosis assay by flow cytometry in HBE cells transfected with SUR1 siRNAs and treated with TNF-α. Data are presented as mean ± SD ( n = 3). ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001. Co-localization in (B) was assessed using Pearson’s correlation coefficient. Data in (C–F) were analyzed using two-way ANOVA followed by Tukey’s multiple-comparisons test. Representative images are shown.

    Article Snippet: Human embryonic kidney cell line HEK293T , American Type Culture Collection , N/A.

    Techniques: Co-Immunoprecipitation Assay, Western Blot, Transfection, Immunofluorescence, Staining, Homologous Recombination, Reporter Assay, Flow Cytometry, Analysis, Knockdown, Apoptosis Assay

    Journal: iScience

    Article Title: Sulfonylurea receptor 1 deficiency alleviates acute lung injury by activating PARP1 in epithelial cells

    doi: 10.1016/j.isci.2026.117627

    Figure Lengend Snippet:

    Article Snippet: Human embryonic kidney cell line HEK293T , American Type Culture Collection , N/A.

    Techniques: Cell-Signaling, Recombinant, Transfection, Caspase-3 Activity Assay, Protein Extraction, Knock-Out, Sequencing, Control, Plasmid Preparation, Software, Analysis